F2442 Fetal Bovine Serum. Unless otherwise stated cells were maintained in DMEM (CellGro 10017CV Corning) supplemented with 10% fetal bovine serum (FBS F2442 SigmaAldrich) Cell lines were cultured in a 37 °C.
Fbs Sigma Aldrich from sigmaaldrich.com
OptiMEM ® (Thermo Fisher Scientific Gibco ™ catalog number 31985070) or serum free medium (SFM appropriate culture medium before adding fetal bovine serum) Phosphate buffered saline (PBS) (GE Healthcare catalog number SH30256) Trypsin solution (25% wt/vol) (GE Healthcare catalog number SH3004201).
